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Image Search Results
Journal: Frontiers in Oncology
Article Title: Multi-Faceted Effects of ST6Gal1 Expression on Precursor B-Lineage Acute Lymphoblastic Leukemia
doi: 10.3389/fonc.2022.828041
Figure Lengend Snippet: BCP-ALL cells contain high levels of α2-6 sialylation. (A) Western blot of different BCP-ALL cell lines probed with SNA lectin to specifically detect α2-6-linked sialic acids on glycoproteins. GAPDH, loading control. Location of molecular weight standards to the right. (B) Analysis of N-glycans in RS4;11 cells as previously described . Combined results of 15 individual RS4;11 cell samples. Overall, more than 65% of all identified N-glycans were found to be sialylated with 7.4% in α2-3, 14.1% in α2-3/6, and 45.1% in α2-6 attachment.
Article Snippet: BCP-ALL cells were lysed in Triton T-100 lysis buffer with glycerol at pH 7.4 (Alfa Aesar, Cat#J63866AK) and glycoproteins were captured with
Techniques: Western Blot, Molecular Weight